Dados do Trabalho

Título

DIVERSITY OF KINETOPLASTIDA IN NON-HUMAN PRIMATES FROM URBAN FORESTED FRAGMENTS AND WILDLIFE REHABILITATION CENTER AT MIDWESTERN BRAZIL

Introdução

The municipality of Campo Grande, Mato Grosso do Sul, harbors several wild mammal species reported infected by different trypanosomatid species.

Objetivo (s)

Considering that primates have been reported parasitized by Leishmania spp. and Trypanosoma spp., we aimed to evaluate natural infections by trypanosomatids in Sapajus cay and Alouatta caraya in Campo Grande/MS.

Material e Métodos

The animals were captured in three areas: (i) the conservation unit Parque Estadual Mata do Segredo (PEMS); (ii) the anthropized area Instituto São Vicente (ISV); and (iii) the Wildlife Rehabilitation Center (WRC) of Campo Grande. Blood samples were collected for nested Polymerase Chain Reaction (nPCR) of the Trypanosomatidae SSU rRNA and Next Generation Sequencing (NGS) for species identification, while serum samples were collected for serological diagnosis of Trypanosoma cruzi and Leishmania spp.

Resultados e Conclusão

Fifty animals were captured and five recaptures were performed: 11 S. cay + two recaptures in ISV, 27 S. cay + three recaptures in PEMS and eight S.cay and four A. caraya in WRC. The nPCR revealed positivity in 37/55 (67.3%) of the samples. All serological tests were negative. Thirty-two nPCR positive samples were submitted for NGS analysis. Several taxonomic unit sequences of trypanosomatids already recorded infecting primates in the Latin America and trypanosomatids related to other hosts were detected in the primates sampled in the present study. Concerning S. cay, we detected L. infantum, T. cruzi TcII, L. amazonensis and Trypanosoma sp. DID (originally found in marsupials of the genus Didelphis) in all three sampled areas; T. cruzi TcI in PEMS and in ISV; T. cruzi TcIV in PEMS and WRC; T. minasense in the PEMS and WRC. Furthermore, T. rangeli and Bodo sp. were detected only in ISV and T. lainsoni (parasite related to small mammals) only in WRC. Regarding A. caraya, we detected T. cruzi (three characterized as TcIV and one as TcII/TcIV), T. minasense, Trypanosoma sp. DID, L. amazonensis and L. infantum. The restriction of its home-range, greater proximity to domestic animals and humans may favor an increase of infections by multi-host parasites (as Leishmania and Trypanosoma species) in non-human primates that inhabit urban forest fragments.

Palavras-chave

Molecular detection, Reservoir system, Trophic web, Brazilian Middle western, Sapajus cay, Alouatta caraya.

Agradecimentos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior e Conselho Nacional de Desenvolvimento Científico e Tecnológico (CAPES); FAPERJ