Dados do Trabalho

Título

Fungi microbiota screening of Tropidurus sp. in the caatinga biome of Piauí, Brazil.

Introdução

Tropidurus sp. is a genera of terrestrial ,oviparous, reptile with diurnal habits and it is found in several biomes, including the Caatinga. The evaluation of the fungi microbiota of these animals can help to understand their role in the dispersion of fungi in the environment.

Objetivo (s)

The objective of the study was the isolation and identification of fungi from the microbiota of Tropidurus sp. and the soil where they live by means of polyphase taxonomy.

Material e Métodos

The samples were collected in the city of São Raimundo Nonato, Piauí, and sent to the LTBBF/IOC/FIOCRUZ, a total of 5 swabs (ASw) and 5 soil samples (ASo) were done. The swabs of the oral, cloacal and interdigital cavities of the animals were inoculated in Agar Sabouraud Dextrose (SDA) medium with Chloramphenicol, and the soil samples were processed by the serial dilution technique in sterile H2O until the 10-3 dilution and inoculated in plates of medium SDA and Potato Dextrose Agar.
After 3 days in a incubator at 25ºC , it was performed the macroscopic analysis and one colony of each distinctive macromorphology was inoculated in SDA medium for the isolation of a single colony. After 7 days, it was observed the macro and microscopic characteristics in order to obtain its taxonomic identification.

Resultados e Conclusão

18 fungal isolates from the evaluated lizards and 42 fungal ones from the soil were obtained. The isolates were identified such as: Aspergillus sp. (4ASw, 13ASo), Mycelia sterilia (5ASw, 2ASo), Penicillium sp. (4ASw,21ASo), Trichoderma sp. (1ASw,2ASo), Fusarium (1ASo), Purpureocillium sp. (2ASo), fungi of the Demateaceae family (2ASw, 1Aso), and fungi of the phyla Zygomycete (2ASw) and yeasts (2ASw, 25ASo).
The initial results reflect the importance of continuous monitoring of the fungal species found in the microbiota of this wild lizard and its relationship with the soil microbiota. Human contact with this animal due to the expansion of agricultural areas, as well as its role as food source, highlights this animal as an important biological vector in regard to public health, because some species detected in its microbiota are fungi with pathogenic potential.

Palavras-chave

Fungi; Tropidurus; Microbiota