Dados do Trabalho

Título

Enhancing Local Genomic Surveillance of SARS-CoV-2 in Mato Grosso do Sul, Central Brazil: Collaborative Insights from University and Public Healthcare Services

Introdução

Collaborative insights often emerge from the collective efforts of diverse stakeholders who contribute unique perspectives, data, and experiences, leading to a more comprehensive and holistic understanding of specific subjects or issues. Within the context of the COVID-19 pandemic, collaborative insights shed light on the lessons learned and knowledge gained through the joint work of universities and healthcare services in implementing genomic surveillance of SARS-CoV-2 in Brazil.

Objetivo (s)

This study presents data from a local genomic surveillance network for SARS-CoV-2 in Mato Grosso do Sul (MS), Brazil, comprising the Federal University of Mato Grosso do Sul and the State Health Secretariat of MS, covering the period from 2020 to 2022.

Material e Métodos

A total of 371 samples of SARS-CoV-2 positive cases were subjected to Whole Genomic Sequencing using the Ion GeneStudioS5 platform (Thermo Fisher Scientific, USA) or the MinION platform (Oxford Nanopore Technologies) between May 2020 and November 2022.

Resultados e Conclusão

In the three years of genomic surveillance, 43 SARS-Cov-2 lineages were identified, and it was possible to observe a change in the predominance of lineages over time. In 2020 only five lineages were identified (B.1.1.28, N.4, B.1.1.33, B.1.1.44, P.2), and P.2 lineage was the most abundant. In 2021 five lineages were also identified (B.1.1.28, P.1, P.2, A.2.5.2, and P.1.2), with P.1 lineage becoming more abundant. Finally, in 2022 the Omicron Variant of Concern accounted for 100% of the evaluated samples and was classified into 36 sub lineages grouped into 11 major groups, with the BA.2 group being the most prevalent, followed by the BA.1 group and the BA.5 group. Even bordering two countries, Bolivia and Paraguay, changes in the genomic profile of MS over the years have followed a similar pattern to the rest of Brazil. Taken togheter, institutional collaboration played a pivotal role in the regionalization of the sequencing process, ensuring timely access to surveillance information in line with the principles of the Unified Health System. Moreover, this collaborative effort proved crucial in developing human resources and facilitating streamlined decision-making among managers.

Palavras-chave

Covid-19, SARS-CoV-2, Genomic Surveillance

Agradecimentos

Secretaria de Estado de Saúde de Mato Grosso do Sul (Projeto FUNDECT 71/015.984/2022). Laboratórios De Campanha para o Diagnóstico de Covid-19 (Rede Vírus/MCTI/FINEP)