Dados do Trabalho

Título

Detection of Mansonella spp in blood samples from inhabitants of the municipalities of Leticia and Puerto Nariño - Amazonas (Colombia)

Introdução

The department of Amazonas in Colombia has the largest territorial extension, and is part of the countries that make up the pan-Amazon and borders on the west with Brazil and on the west with Peru. Filariasis are currently part of the neglected infectious diseases in Colombia and are generally not considered a public health problem, due to the lack of knowledge of the causes and consequences of the disease, as well as its relationship with the environment. In the Amazon, this phenomenon becomes even more complex due to the difficulties of access, the great distances and the different situations of vulnerability of the riverside populations, adding the constant transit of people and the floating population that carry a greater risk of (re)emergence and spread of pathogens.

Objetivo (s)

Detect the presence of Mansonella spp in riverside inhabitants of the municipalities of Leticia and Puerto Nariño - Amazonas (Colombia) through molecular diagnosis of the filarial species present in the collected blood samples.

Material e Métodos

The blood samples collected for the study come from the riverside communities of the municipalities of Leticia and Puerto Nariño. This is a descriptive, observational, cross-sectional study; For the detection of microfilariae, the Nested PCR molecular and the DNeasy Blood & Tissue Kit was used, according to the methodology described by Tang et al., (2010).

Resultados e Conclusão

To date, 14 blood samples from the municipalities of Leticia and Puerto Nariño were collected and processed; 50% of the samples (only of Puerto Nariño) were positive for Mansonella ozzardi by thick blood smear microscopy, with low parasitemia. The results by PCR Nested and by genetic sequencing confirmed the presence of Mansonella ozzardi in the positive samples by microscopy. It is necessary to carry out a basic survey of the ecoepidemiology of this neglected disease that helps to strengthen cross-border surveillance and laboratory diagnosis.

Palavras-chave

border, cross-border surveillance, filariasis, Mansonella, neglected diseases

Agradecimentos

Research Nucleus of Pathogens, Reservoirs and Vectors in the Amazon – PReV Amazônia/EDTA, Leónidas and Maria Deane Institute – ILMD/Fiocruz Amazônia. ESE Hospital San Rafael de Leticia and the Local Hospital of Puerto Nariño, Amazonas, Colombia.